Renal Disease & Injury

Renal dysfunction resulting from acute kidney injury (AKI) and chronic kidney disease (CKD) is a prevalent clinical condition for which there is a need for effective preventative and therapeutic strategies. Whereas AKI is typically abrupt in nature, CKD develops gradually over time causing a progressive deterioration in renal function. Although temporally distinct, AKI and CKD are closely related and interconnected, with each syndrome representing a risk factor for the other. Furthermore, both AKI and CKD are linked to other clinical disorders such as hypertension and heart disease resulting in cardiorenal syndrome.  PBI offers a wide array of animal paradigms that model human conditions of AKI and CKD, including acute renal failure, acute and chronic renal ischemia/reperfusion, nephrotoxicity, and diabetic nephropathy.

Acute Kidney Injury & Failure (AKI / ARF)

Model Species Top Tier Data
 Unilateral Ureter Occlusion (UUO)  Rat & Mouse
  • Renal Histology (fibrosis)
  • Renal Gene Expression
  • Kidney Cortex Hydroxyproline
 Renal Ischemia/Reperfusion (I/R)  Rat & Mouse
  • Blood Pressure (direct and indirect)
  • Creatinine Clearance, GFR
  • Urinary Protein, KIM1, NGAL, Albumin, MCP-1, Osteopontin
  • Serum Creatinine, BUN, Electrolytes
 Glycerol Induced Rhabdomyolysis  Rat

Nephrotoxicity

Model Species Top Tier Data

Cisplatin

Mouse

  • Creatinine Clearance
  • Urinary Protein, KIM1, NGAL, Albumin, MCP1
  • Serum Creatinine, BUN

Acute Streptozotocin

Mouse

Puromycin (PAN)

Mouse

Chronic Kidney Disease & Renal Insufficiency

Model Species Top Tier Data

Renal Ischemia/Reperfusion

Rat & Mouse

  • Creatinine Clearance, GFR
  • Urinary Protein, KIM1, NGAL, Albumin
  • Serum Creatinine, BUN

5/6 NPX + NaCl (pole ablation method)

Rat & Mouse

  • Blood pressure (Direct & Indirect)
  • Creatinine Clearance, GFR
  • Urinary Protein, KIM1, NGAL, Albumin, MCP-1
  • Serum Creatinine, BUN, Electrolytes
  • Plasma NT-proBNP, NT-proANP

5/6 NPX (renal infarction method)

Rat

DOCA + Salt

Rat

ANGII + L-NAME

Rat

Autoimmune Kidney Injury

Model Species Top Tier Data

Nephrotoxic Serum Nephritis

(Anti-Glomerular Basement Membrane Ab)

Mouse
  • Creatinine Clearance
  • Urinary Protein, KIM1, NGAL, Albumin
  • Serum Creatinine, BUN

Passive Heymann Nephritis (Anti-Fx1A ab)

Rat

Diabetic Nephropathy

Model Species Top Tier Data

ZSF-1 (±NaCl, ±NPX)

Rat
  • Blood Pressure (Direct & Indirect)
  • Creatinine Clearance, GFR
  • Urinary Protein, KIM1, NGAL, Osteopontin Albumin
  • Serum Creatinine, Bun, Electrolytes Triglycerides, Glucose, HbA1C
  • Kidney Histology (fibrosis, macrophages)
  • Kidney Profibrotic & Proinflammatory mRNA Expression
  • Cardiac Histology (Fibrosis, macrophages)
  • Cardiac Gene Expression
  • Echocardiography

Zucker Diabetic Fatty (ZDF) Rat

Rat

db/db Mouse (±NPX)

Mouse

BtBr Mouse

Mouse

Streptozotocin (±NPX)

Mouse

(Rat – Under Development)